San Diego Anti-aging medicine and family practice located in Encinitas CA, Center for Age Management

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Center for Age Management

317 N. El Camino Real, Suite 206
Encinitas, CA 92024
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Phone: 760-633-1315


Mon - Thur: 9am to 5pm
Lunch: 12pm to 1:30pm
Friday: 9am to 12pm
On Fridays, we will have limited staff. You may leave a message and it will be our pleasure to return your call. Any prescriptions, questions or concerns that you have, we will be happy to assist you Monday - Thursday.

Serving other neighboring cities: La Costa, Solana Beach, Rancho Santa Fe, Del Mar, San Marcos, Carlsbad, La Jolla, and San Diego County, CA.

Effects of Progesterone and 18-Methyl Levonorgestrel on Osteoblastic Cells

Objective. To study the effects of progesterone (P) and 18-methyl levonorgestrel (LNG) on the expression of P receptors (PRA and PRB), c-fos, c-jun, and osteocalcin in the human osteosarcoma cell line MG-63 and human normal osteoblasts in order to understand the mechanism of progestin action on the proliferation and differentiation of osteoblasts. Methods. Cell proliferation was tested by MTT assay. The expression of PR, c-fos, c-jun, and osteocalcin was measured by semi-quantitative RT-PCR, Western immunoblot or immunocytochemistry. Results. Progesterone and LNG (10-10-10-6 M) stimulated cell proliferation in a dose-dependent manner. Progesterone and LNG did not affect the expression of PRA and PRB mRNA and protein, but c-fos and c-jun mRNA and protein were upregulated in a dose-dependent manner. The expression of osteocalcin mRNA was also increased in human osteoblasts in a dose and time-dependent manner with greater effects of LNG than P, while the expression of osteocalcin mRNA in MG-63 cells was not changed by P or LNG. Conclusion. Progesterone and LNG promote osteocalcin gene transcription by stimulating the expression of c-fos and c-jun, and result in osteoblast proliferation and differentiation.

Keywords: Progesterone; 18-methyl levonorgestrel; Human osteoblasts; Human osteosarcoma cell line MG-63; Progesterone receptor; C-fos; C-jun osteocalcin

Authors: Min Liang a; Er-yuan Liao a; Xin Xu a; Xiang-hang Luo a; Xin-hua Xiao a
Affiliation: a Institute of Endocrinology & Metabolism, The Second Xiangya Hospital of Central South University. Changsha, Hunan. P.R. China
DOI: 10.1081/ERC-120026954
Publication Frequency: 4 issues per year
Published in: Endocrine Research, Volume 29, Issue 4 January 2003 , pages 483 – 501
Subject: Endocrinology;